Is intra articular injection of triamcinolone acetonide a better option in management for primary osteoarthritis knee than methylprednisolone acetate?

Background: Intra articular corticosteroid injections are commonly using in management of osteroarthritis of knee. Most commonly used ones are methylprednisolone acetate (MPA) and triamcinolone acetonide (TA). The aim of our study is to compare the efficacy of these two agents in treating osteoarthritis of knee and find out if any one agent is superior to other if so.Methods: Selected patients with symptomatic OA knee with kellgren-Lawrence grade III were given intra articular steroid injections (40 mg TA or 40 mg MPA) and were reassessed on day 0, 1 month, 3 months and 6 months. VAS and Knee society scores were calculated using questionnaire method and compared the scores.Results: The VAS and KSS was improved significantly on day 0,1 month and 6th month and it was found to be increased at 6th month in both the groups and the values were comparable in both the groups. The effect of both the agents last for 3-6 months and the effects found to be maximum at 1st month and the effects starts to wean off at the end of 6 months in both the groups.Conclusions: Both methylprednisolone acetate (MPA) and triamcinolone acetonide (TA) are equally effective in reducing pain in patients with osteoarthritis of knee and thereby improving their functional ability upto 6 months

Co-translational assembly of proteasome subunits in NOT1-containing assemblysomes

The assembly of large multimeric complexes in the crowded cytoplasm is challenging. Here we reveal a mechanism that ensures accurate production of the yeast proteasome, involving ribosome pausing and co-translational assembly of Rpt1 and Rpt2. Interaction of nascent Rpt1 and Rpt2 then lifts ribosome pausing. We show that the N-terminal disordered domain of Rpt1 is required to ensure efficient ribosome pausing and association of nascent Rpt1 protein complexes into heavy particles, wherein the nascent protein complexes escape ribosome quality control. Immunofluorescence and in situ hybridization studies indicate that Rpt1- and Rpt2-encoding messenger RNAs co-localize in these particles that contain, and are dependent on, Not1, the scaffold of the Ccr4-Not complex. We refer to these particles as Not1-containing assemblysomes, as they are smaller than and distinct from other RNA granules such as stress granules and GW- or P-bodies. Synthesis of Rpt1 with ribosome pausing and Not1-containing assemblysome induction is conserved from yeast to human cells